Coding
Part:BBa_K4060021:Design
Designed by: Yan-Zhen, Chen Group: iGEM21_NYCU-Taipei (2021-09-30)
pelB leader sequence with aprN (without native signal peptide sequence)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 67
Illegal NotI site found at 211 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 67
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 345 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 349
Design Notes
We replace the native signal peptide sequence of aprN with pelB leader sequence by PCR, restriction enzyme digestion and ligation. With pelB leader sequence, it can increase the secretion of Nattokinase.
Source
Please refer to iGEM part: BBa_J32015 and BBa_K4060020